Sequencing of Genomic DNA by Combined Amplification and Cycle Sequencing Reaction
نویسندگان
چکیده
منابع مشابه
Sequencing of genomic DNA by combined amplification and cycle sequencing reaction.
BACKGROUND Despite considerable advances, DNA sequencing has remained somewhat time-consuming and expensive, requiring three separate steps to generate sequencing products from a template: amplification of the target sequence; purification of the amplified product; and a sequencing reaction. Our aim was to develop a method to routinely combine PCR amplification and cycle sequencing into one sin...
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ژورنال
عنوان ژورنال: Clinical Chemistry
سال: 2005
ISSN: 0009-9147,1530-8561
DOI: 10.1373/clinchem.2004.039164